Journal: Frontiers in Endocrinology

Article Title: Diagnostic Role of Prostate-Specific Membrane Antigen in Adrenocortical Carcinoma

doi: 10.3389/fendo.2019.00226

Figure Lengend Snippet: The staining of PSMA and CD34 in ACA and ACC (X200). As we can see from the figure, the staining of PSMA in CPA, APA, NFT and metastases were negative, while in ACC it was positive with high percentage.

Article Snippet: The slides were stained for CD34 (1:200; ZM-0046; Origene) and PSMA (diluted 1:100; ab19071; Abcam).

Techniques: Staining

Journal: Journal of Nanobiotechnology

Article Title: Cerium oxide nanoparticles-carrying human umbilical cord mesenchymal stem cells counteract oxidative damage and facilitate tendon regeneration

doi: 10.1186/s12951-023-02125-5

Figure Lengend Snippet: Characterization of hUCMSCs. A Primary hUCMSCs were spindle-shaped under light microscopy. B Flow cytometry analysis showed that 98.5% of cells expressed CD29, 97.4% expressed CD44, 98.6% of cells expressed CD90, and 98.1% of cells expressed CD105. Meanwhile, only 0.48% and 0.28% expressed CD45 and CD34. C Lipid droplets were stained by oil red O after adipogenic differentiation induction of hUCMSCs. Mineralized nodules were stained by Alizarin Red after osteogenic differentiation induction of hUCMSCs. Acid mucopolysaccharide was stained by Alcian Blue after chondrogenic differentiation induction of hUCMSCs

Article Snippet: Briefly, 10 7 hUCMSCs were subjected to analysis for surface markers CD34 (E-AB-F1143D, Elabscience), CD44 (E-AB-F1100D, Elabscience), CD45 (E-AB-F1137D, Elabscience), CD29 (E-AB-F1049D, Elabscience), CD90 (E-AB-F1167D, Elabscience), and CD105 (E-AB-F1310D, Elabscience) by flow cytometry following the manufacturer’s protocol.

Techniques: Light Microscopy, Flow Cytometry, Staining

Journal: Molecular Therapy Nucleic Acids

Article Title: Ligand-modified rAAV6 vectors with nanoblades allow high level gene knock-in in HSPCs without compromising cell survival

doi: 10.1016/j.omtn.2025.102495

Figure Lengend Snippet: Figure 5. Coupling of rAAV6 with low amounts of mannose allowed high-level nanoblade-mediated knockin CD34+ cells without cell death induction (A) 8E4, 3E5, or 8E5 mannose sugars were incubated for 4 h at RT with 10E12 vg of rAAV6 vectors encoding for the donor template. 10E9 vg rAAV6 vector per condition were analyzed in dot blot using the ADK1 antibody for rAAV6 that recognizes the intact assembled capsid or conca- navalin A, which detected mannose bound to the capsid. (B) 5E8 vg of rAAV6 conjugated with indicated quantities of mannose were separated on a gel, followed by silver staining to reveal the intact capsids. (C and D) CD34+

Article Snippet: Purity of the selected CD34+ cell fraction was evaluated by fluorescence-activated cell sorting (FACS) analysis (FACSCanto, BD) with APC-conjugated anti-CD34 antibody (Miltenyi Biotech).

Techniques: Knock-In, Incubation, Plasmid Preparation, Dot Blot, Silver Staining

Journal: Stem Cell Research & Therapy

Article Title: Iberian pig mesenchymal stem/stromal cells from dermal skin, abdominal and subcutaneous adipose tissues, and peripheral blood: in vitro characterization and migratory properties in inflammation

doi: 10.1186/s13287-018-0933-y

Figure Lengend Snippet: Analysis by flow cytometry of expression levels of cell surface markers CD34, CD44, CD105, CD90 and MHCII and intracellular markers cytokeratin, vimentin and POU5F1 in AA-MSCs, DS-MSCs, SCA-MSCs and PB-MSCs. Data correspond to mean fluorescence intensity (fold of negative control) for each sample. AA-MSC abdominal adipose tissue mesenchymal stem/stromal cell, DS-MSC dermal skin tissue mesenchymal stem/stromal cell, MHCII major histocompatibility complex II, PB-MSC peripheral blood mesenchymal stem/stromal cell, POU5F1 POU class 5 homeobox 1, SCA-MSC subcutaneous adipose tissue mesenchymal stem/stromal cell

Article Snippet: Appropriate dilutions, provided by manufacturers, of primary antibodies against the markers commonly used to define MSCs—vimentin (clone LN-6; Sigma-Aldrich), CD44 (clone IM7; Bio-rad), CD105 (clone MEM-229; Abcam) and CD90 (clone 5E10; Abcam) as positive markers, cytokeratin, CD34 (rabbit polyclonal; Biorbyt) and MHCII (clone CVS20; Bio-Rad) as negative markers and POU5F1 as a pluripotency marker—were added to the cells and incubated overnight at 4 °C.

Techniques: Flow Cytometry, Expressing, Fluorescence, Negative Control, Immunopeptidomics

Journal: Cellular & molecular biology letters

Article Title: Reciprocal negative feedback between Prrx1 and miR-140-3p regulates rapid chondrogenesis in the regenerating antler.

doi: 10.1186/s11658-024-00573-x

Figure Lengend Snippet: Fig. 4 Characteristics of tissue layers of the antler growth center and isolated RM cells. A Immunofluorescence assay of CD73, CD90, Nestin, CD34, and Prrx1 (red color) in the RM, PC, and CA layers, respectively. B Third passage of cultured cells from the RM layer were identified using mesenchymal stem cell markers: CD73 and CD90 (red), Nestin and CD34 (green), respectively; nuclei were stained blue with DAPI. C Chondrogenic differentiation of RM cells, Alcian blue, and Col II immunofluorescence staining. D Osteogenic differentiation of RM cells with Alizarin red staining. E Adipogenic differentiation of RM cells with Oil Red O staining (scale bars, 125 μm and magnification ×200)

Article Snippet: The cells and tissues were fixed, permeabilized, blocked, and incubated overnight with primary antibodies: CD90 (1:300, proteintech, USA, 66766-1-lg), CD73 (1:1000, proteintech, USA, 12231- 1-AP), Nestin (1:300, BIOSS, China, bs-0008R), CD34 (1:300, proteintech, USA, 14486- 1-AP), and Prrx1 (1:200, Absin, China, abs134576).

Techniques: Isolation, Immunofluorescence, Cell Culture, Staining